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. 2013 Apr 30;8(4):e61901. doi: 10.1371/journal.pone.0061901

Figure 5. DNMT1 is linked to activated IL-6 signaling via Akt phosphorylation.

Figure 5

A. IL-6 level was positively correlated with DNMT1 expression in human bladder cancer specimens (P<0.0001). Representative slides of a selected tumor specimen positively staining for both IL-6 and DNMT1, and another tumor specimen negative for both IL-6 and DNMT1 are shown at ×400 magnification. B. Effect of IL-6 on the level of DNMT1, p-AKT, and p-STAT3 was examined by Western blotting (CV, cells transfected with the control vector; IL-6 SV, cells transfected with the IL-6 silencing vector). C. Effect of IL-6 on the level of DNMT1 and p-AKT was evaluated by immunohistochemical staining, and the results from representative slides are shown. D. Effect of IL-6 inhibition by IL-6-neutralizing antibody, p-AKT inhibition by PI3K inhibitor, and p-STAT3 inhibition by STAT3 siRNA on the level of DNMT1 was examined by Western blotting (W, wild-type; IL-6−, cells treated with IL-6-neutralizing Ab; STAT3−, cells treated with Stat3 siRNA; PI3K-I, cells treated with PI3K inhibitor.