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. 2013 Apr 30;8(4):e63179. doi: 10.1371/journal.pone.0063179

Figure 1. IGF-1 induces MDM2 expression in a PI3-Kinase-dependent pathway.

Figure 1

(A) U2-OS cells were serum-starved for 24 hours and then pre-treated with either PI3-kinase (PI3K) inhibitor LY294002 (20 µM), MAP kinase kinase (MEK) inhibitor PD98059 (25 µM), or p38 stress-activated protein kinase inhibitor SB202190 (10 µM) for four hours, followed by treatment with IGF-1 (50 ng/mL) for six hours. Cells were lysed and subjected to western blot analysis. (B) H1299 cells were serum-starved by incubating in the absence of FBS for 24 hours and treated with IGF-1 for six hours as indicated. Cell lysates were subjected to western blot analysis. S: short exposure; L: long exposure.