Table 1. Improvement of PCR and sequencing success rates using ITS1 mini-barcodes.
Genus | PCR Positive | Sequence Positive |
Cortinarius | 30/30 (100%) | 24/30 (80%) |
Russula | 30/30 (100%) | 27/30 (90%) |
Mycena | 27/30 (90%) | 4/30 (13%) |
PCR and sequencing success rates are shown for 30 randomly-selected samples from 3 macrofungal genera; samples were previously negative for full-length ITS1+5.8S+ITS2 amplification.