(A–C) Fluorescently labeled VLE RNA was microinjected into oocytes expressing (A) p150Glued CC1 domain, (B) p50-dynamitin, or (C) no exogenous protein. (D) Quantification of in vivo interference results (control [control, n = 69], p150Glued CC1 [CC1, n = 97], and p50-dynamitin [Dyna., n = 98]). Black bars indicate normal localization; gray denotes cup accumulation. Error bars indicate standard deviation. (E) Oocytes microinjected with fluorescently labeled VLE RNA were probed with anti-dynein. Shown is a confocal section with dynein in green (E), VLE RNA in red (E′), and co-localization in yellow (E″). (F) Zoomed view of (E″) showing the vegetal cytoplasm. (A–F) Representative confocal images of fixed oocytes are shown, with the vegetal pole towards the bottom. Scale bars, 50 µm.