Table 5.
Differentially regulated miRs and their validated target genes in pNK, compared to PB NK.
| miR | Experimentally validated genes | |||||
|---|---|---|---|---|---|---|
| HIGHLY EXPRESSED MIRNAS IN PB NK | ||||||
| hsa-let-7a | TRIM71 (7) | HMGA1 (4) | LIN28A (3) | ACP1 (2) | E2F2 (2) | SMOX (1) |
| HMGA2 (6) | THBS1 (4) | CASP3 (3) | RTCD1 (2) | ITGB3 (2) | MYC (1) | |
| UHRF2 (5) | NRAS (3) | PRDM1 (2) | CCND2 (2) | LIN28 (1) | TUSC2 (1) | |
| MED28 (4) | EIF2C4 (3) | DICER1 (2) | SLC20A1 (2) | NF2 (1) | BCL2 (1) | |
| ZFP36L1 (1) | NKIRAS2 (1) | EGR3 (1) | IL6 (1) | NEFM (1) | ||
| hsa-let-7b* | HMGA2 (6) | CDC25A (3) | GRPEL2 (2) | NXT2 (2) | CDIPT (1) | SLC25A13 (1) |
| IGF2BP1 (5) | AURKB (3) | MARS2 (2) | EIF2C3 (2) | CDKAL1 (1) | SLC25A1 (1) | |
| TMEM2 (5) | DHX57 (3) | MRM1 (2) | CCNA2 (2) | CSNK1D (1) | UHRF1 (1) | |
| LIN28B (5) | FNDC3A (3) | POM121 (2) | CCNF (2) | DOCK5 (1) | C20ORF72 (1) | |
| CCNJ (5) | RDH10 (3) | PXDN (2) | EDEM3 (2) | FADS2 (1) | SCAMP3 (1) | |
| CDC34 (5) | SLC25A24 (3) | SCYL1 (2) | TRABD (2) | FAM96A (1) | C2ORF18 (1) | |
| IGF2BP2 (5) | SNAP23 (3) | SLC25A32 (2) | PLAGL2 (2) | GPR56 (1) | CIAO1 (1) | |
| DMD (5) | LIN28A (3) | SPRYD4 (2) | FARP1 (2) | IPO4 (1) | BIRC6 (1) | |
| E2F6 (5) | PRDM1 (3) | TAF9B (2) | C7ORF58 (2) | KIAA0409 (1) | AURKA (1) | |
| HMGA1 (4) | NRAS (3) | TTC9C (2) | LIN28 (1) | NEDD4 (1) | ALG3 (1) | |
| PGRMC1 (4) | RRM2 (3) | DLC1 (2) | BCL7A (1) | OPRS1 (1) | ARID3A (1) | |
| THBS1 (4) | CCND1 (2) | CCND2 (2) | ACTG1 (1) | RHOB (1) | CCBL2 (1) | |
| PDE12 (4) | ATP6V1F (2) | DICER1 (2) | AARSD1 (1) | RHOG (1) | RRP1B (1) | |
| E2F5 (4) | GEMIN7 (2) | GTF2I (2) | ANAPC1 (1) | SLC1A4 (1) | TAB2 (1) | |
| hsa-let-7g | HMGA2 (6) | EIF4G2 (5) | IGF2BP1 (5) | COL1A2 (3) | BCL2L1 (1) | |
| hsa-miR-10a | NCOR2 (5) | MAP3K7 (4) | HOXA1 (1) | USF2 (1) | BTRC (1) | |
| hsa-miR-133b | BCL2L2 (3) | MCL1 (2) | ||||
| hsa-miR-146b* | TRAF6 (7) | IRAK1 (5) | MMP16 (2) | CARD10 (1) | ||
| hsa-miR-152 | DNMT1 (5) | HLA-G (2) | ||||
| hsa-miR-155 | SOCS1 (5) | DET1 (4) | SPI1 (3) | SMAD1 (2) | TM6SF1 (1) | RAB34 (1) |
| TSHZ3 (5) | IKBKE (3) | MEIS1 (2) | TRAM1 (2) | FOXO3 (1) | RAB6A (1) | |
| HIVEP2 (5) | PHF17 (3) | SMAD2 (2) | TRIP13 (2) | ARL5B (1) | SYPL1 (1) | |
| TAB2 (4) | BACH1 (3) | CYR61 (2) | FGF7 (2) | ATG3 (1) | VAMP3 (1) | |
| JARID2 (4) | RCN2 (3) | TP53INP1 (2) | KRAS (2) | ATP6V1C1 (1) | WDFY1 (1) | |
| CEBPB (4) | RCOR1 (3) | ANKFY1 (2) | HIF1A (2) | BET1 (1) | ETS1 (1) | |
| ARID2 (4) | ZNF652 (3) | CHAF1A (2) | C5ORF41 (2) | CBFB (1) | INPP5D (1) | |
| DHX40 (4) | MYB (3) | CLDN1 (2) | IKBIP (2) | DNAJB1 (1) | PAPOLA (1) | |
| PICALM (4) | TLE4 (3) | MYO10 (2) | TWF1 (2) | DSG2 (1) | SERTAD2 (1) | |
| TRIM32 (4) | CSF1R (3) | NARS (2) | CUX1 (2) | FMNL2 (1) | ERMP1 (1) | |
| ZIC3 (4) | FAR1 (3) | PHC2 (2) | SLA (2) | PKN2 (1) | C3ORF58 (1) | |
| KBTBD2 (4) | ZNF236 (3) | SDCBP (2) | AGTR1 (1) | PRAF2 (1) | HNRNPA3P1 (1) | |
| MSH2 (1) | PELI1 (1) | |||||
| hsa-miR-17-5p | E2F1 (1) | |||||
| hsa-miR-181b | GRIA2 (4) | GATA6 (4) | TIMP3 (4) | MAP3K10 (3) | NLK (2) | PLAG1 (2) |
| CYLD (2) | VSNL1 (1) | KAT2B (1) | BCL2 (1) | |||
| hsa-miR-181c | GATA6 (4) | KRAS (3) | NLK (2) | NOTCH4 (2) | NOTCH2 (1) | |
| hsa-miR-181d | GATA6 (3) | NLK (2) | BCL2 (1) | |||
| hsa-miR-186 | AKAP12 (1) | |||||
| hsa-miR-191 | TMC7 (2) | SOX4 (1) | ||||
| hsa-miR-197 | FBXW7 (4) | DPH1 (2) | UMPS (2) | CLIC1 (1) | HNF4A (1) | FOXO3 (1) |
| CHIC2 (4) | ALMS1 (2) | CPNE6 (2) | WDR6 (1) | PEX13 (1) | IL1R1 (1) | |
| ACVR1 (4) | CES1 (2) | RBM4 (2) | NEK4 (1) | C1ORF38 (1) | CPSF1 (1) | |
| RAB28 (3) | ZNF302 (2) | CYLD (2) | PIPOX (1) | MED16 (1) | SNX1 (1) | |
| HNRNPD (2) | RAD51 (2) | RFX1 (2) | IGF2AS (1) | LRP4 (1) | KLF10 (1) | |
| GOLGB1 (2) | RXRB (2) | IER3 (1) | DCBLD2 (1) | TSPYL1 (1) | AGR2 (1) | |
| TUSC2 (1) | EHD2 (1) | |||||
| hsa-miR-199b | LAMC2 (1) | HES1 (1) | ||||
| hsa-miR-200c | ZFPM2 (6) | ZEB1 (5) | ERRFI1 (5) | ZEB2 (5) | FN1 (5) | UBE2I (3) |
| BAP1 (2) | PTPN13 (1) | BMI1 (1) | JAG1 (1) | TUBB3 (1) | ||
| hsa-miR-21 | TGFBI (5) | GLCCI1 (3) | RASGRP1 (3) | SGK3 (2) | ANKRD46 (2) | SLC16A10 (1) |
| NFIB (4) | SOX5 (3) | MSH2 (3) | RP2 (2) | ACTA2 (1) | TIMP3 (1) | |
| RECK (4) | JAG1 (3) | PCBP1 (3) | SERPINB5 (2) | BTG2 (1) | TGFBR2 (1) | |
| PDCD4 (3) | BMPR2 (3) | TOPORS (3) | SPRY2 (2) | SESN1 (1) | NCAPG (1) | |
| FAM3C (3) | TIAM1 (3) | APAF1 (2) | RHOB (2) | SOCS5 (1) | IL1B (1) | |
| RTN4 (1) | PTX3 (1) | CDK2AP1 (1) | ||||
| hsa-miR-222 | CDKN1B (5) | FOS (5) | KIT (4) | CDKN1C (3) | PPP2R2A (2) | MMP1 (1) |
| SOD2 (1) | BBC3 (1) | PTEN (1) | ICAM1 (1) | ESR1 (1) | ||
| hsa-miR-24* | CDKN1B (4) | TRIB3 (4) | MAPK14 (2) | FURIN (2) | BRCA1 (1) | NOTCH1 (1) |
| ACVR1B (4) | DND1 (3) | NFAT5 (2) | MLEC (2) | KIAA0152 (1) | CDKN2A (1) | |
| KHSRP (1) | HNF4A (1) | TGFB1 (1) | ||||
| hsa-miR-26a | SMAD1 (5) | HMGA1 (5) | CDK8 (4) | HMGA2 (3) | CCND2 (2) | CCNE2 (2) |
| STRADB (5) | GSK3B (5) | MTDH (4) | CPEB4 (3) | CTGF (2) | LIF (2) | |
| PTEN (5) | EZH2 (4) | MAP3K2 (4) | SERBP1 (2) | CDC6 (2) | CPEB2 (1) | |
| CPEB3 (1) | SMAD4 (1) | |||||
| hsa-miR-331 | ERBB2 (2) | CDCA5 (2) | KIF23 (1) | |||
| hsa-miR-342 | BMP7 (1) | GEMIN4 (1) | ||||
| hsa-miR-429 | ZFPM2 (6) | ZEB1 (6) | ERRFI1 (4) | ZEB2 (4) | WASF3 (3) | BAP1 (1) |
| HIGHLY EXPRESSED MIRNAS IN PNK | ||||||
| hsa-miR-100 | MTOR (3) | PLK1 (1) | FGFR3 (1) | IGF1R (1) | ATM (1) | |
| hsa-miR-125b | LACTB (6) | BMF (3) | SAMD10 (3) | MKNK2 (2) | BBC3 (2) | DICER1 (1) |
| BAK1 (5) | BMPR1B (3) | EIF4EBP1 (3) | CBFB (2) | QSOX2 (2) | JUB (1) | |
| ARID3B (5) | ENTPD4 (3) | KLF13 (3) | LIN28A (2) | LIN28 (1) | DDX19B (1) | |
| IRF4 (5) | TOR2A (3) | ULK3 (3) | IGF2 (2) | C10ORF104 (1) | PABPC1 (1) | |
| PRDM1 (4) | KCNS3 (3) | SLC7A6 (3) | NKIRAS2 (2) | B3GALT4 (1) | AKT1 (1) | |
| SLC35A4 (4) | LIN28B (3) | SLC7A1 (3) | SEL1L (2) | UBE2I (1) | TP53 (1) | |
| CGN (4) | GRIN2A (3) | ERBB3 (2) | ATXN1 (2) | RBM8A (1) | CASC3 (1) | |
| PPAT (4) | STAT3 (3) | CDKN2A (2) | RAF1 (2) | IGFBP3 (1) | E2F3 (1) | |
| CBX7 (3) | LIF (3) | ABTB1 (2) | CYP24A1 (2) | MAN1A1 (1) | RNF144A (1) | |
| SGPL1 (3) | SMARCD2 (3) | ARID3A (2) | ABCC4 (2) | SMO (1) | LYPLA2 (1) | |
| PLEKHA8 (1) | TP53INP1 (1) | VDR (1) | ||||
| hsa-miR-211 | KCNMA1 (3) | |||||
| hsa-miR-302c | ESR1 (2) | |||||
| hsa-miR-326 | PKM2 (2) | SMO (1) | GLI1 (1) | NOTCH2 (1) | ||
| hsa-miR-422a** | CYP8B1 (2) | |||||
| hsa-miR-519c | HIF1A (4) | |||||
| hsa-miR-519d | CDKN1A (4) | PPARA (3) | ||||
| hsa-miR-520c | APP (3) | CD44 (3) | ||||
| hsa-miR-520g | VEGFA (2) | |||||
| hsa-miR-520h | SMAD6 (3) | ABCG2 (2) | CDKN1A (2) | VEGFA (2) | ID1 (1) | ID3 (1) |
| hsa-miR-522 | SOX2 (1) | |||||
| hsa-miR-342 | BMP7 (1) | GEMIN4 (1) | ||||
| hsa-miR-429 | ZFPM2 (6) | ZEB1 (6) | ERRFI1 (4) | ZEB2 (4) | WASF3 (3) | BAP1 (1) |
*, **Denote that the expression level is significantly higher (2 SD higher) in at least one of the donors than the endogenous control in only the PB or pNK, respectively. Number in parenthesis denotes the number of databases which indicate that the gene is targeted by the miR, in addition to having been validated previously.