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Optimization of ratio of feeder cells: K562 to PBMC.
| Feeder Ratio (K562:PBMC) | Donors (n) | Fold expansion* range (min, max) | Average fold expansion |
|---|---|---|---|
| 1:10 | 9 | 6, 131 | 53 |
| 1:5 | 3 | 11, 54 | 32 |
| 1:1 | 14 | 15, 358 | 98 |
*Fold expansion = absolute number of CD56+ CD3− NK cells on Day 21/absolute number of NK cells on Day 0.
