Fig. 4.

miR-21 knockdown in human pulmonary valve endothelial cells (HPVECs) triggers cell migration. (A) Pre-miR-21 overexpression causes increased HPVEC migration. The relative number of migrating cells (average of three different experiments) shows increased cell migration with TGFβ2 treatment and with miR-21 overexpression. (B) Quantification of western blot showing variation in PDCD4 protein levels under the same conditions as in A. (C) qPCR data demonstrating that PDCD4 mRNA levels do not change under the same conditions as in A. Data are mean ± s.d.; ^*P<0.05.