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. Author manuscript; available in PMC: 2014 Mar 21.
Published in final edited form as: Chem Biol. 2013 Mar 21;20(3):351–359. doi: 10.1016/j.chembiol.2012.12.009

Fig. 1. Screening a combinatorial peptoid library for ligands to NMO-specific antibodies.

Fig. 1

A. Schematic depiction of the screening strategy. An OBOC library was pre-screened with control serum to eliminate the beads that can bind to the antibodies present at high levels in control (non-NMO) sera. The denuded library was then screened against a pool of NMO serum high in anti-AQP4 antibodies. In both the pre-screen and the NMO screen, antibody-binding beads were visualized using a red quantum dot-conjugated secondary antibody. The hits were identified by tandem mass spectrometry. B. General structure of the 8-mer peptoid in the library used for the screening of NMO sera. The invariant linker is shown in black and the variable region in blue with side chains substituents (from the amines) in red. C. List of amines used in the solid-phase synthesis of the library. D. A representative photomicrograph of the library under the fluorescent microscope after incubation with the serum followed by hybridization of anti-human secondary antibody conjugated with Qdot 655. The beads were irradiated through a DAPI filter. The beads with the red halo are the hits.