Skip to main content
. Author manuscript; available in PMC: 2014 Mar 5.
Published in final edited form as: Structure. 2013 Mar 5;21(3):385–393. doi: 10.1016/j.str.2013.01.010

Fig. 3.

Fig. 3

Activity assay results for RNA structure requirement for its processing. (A) Nomenclatures for RNA variants used in the activity assay. Nucleotide changes are indicated by arrows and the resulting mutant RNAs are represented by a single letter. The cleavage site is indicated by a red oval. (B) Radiographs of RNA cleavage reactions with the wild-type and variants of the repeat RNA incubated with 1 μM SsCas6 at 45°C (see Materials and Methods for details).