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. Author manuscript; available in PMC: 2013 Aug 11.
Published in final edited form as: Cancer Cell. 2013 Feb 11;23(2):215–227. doi: 10.1016/j.ccr.2012.12.019

Figure 1. Conditional expression of the human clear cell sarcoma fusion oncogene in the mouse.

Figure 1

(A) Schematic showing the cDNA of the type I variant of EWS-ATF1 isolated from a human tumor and cloned into a vector designed for targeting into the mouse Rosa26 locus. neo-R: neomycin resistance cassette. polyA: polyadenylation stop sequence. IRES : internal ribosome entry site. eGFP: enhanced green fluorescent protein. Cre-mediated recombination excises the stop sequence and initiates expression of the fusion oncogene and eGFP. (B) Embryonic day 14.5 fibroblasts isolated from Rosa26EA1 heterozygous mice were exposed to TAT-Cre protein or vehicle control and images were collected 24 hr later. The left two panels show light images of fibroblasts while the right two panels show the GFP fluorescence. All Scale bars are 50 µm in length.