Figure 2.

Time course of heterosynaptic eCB–LTD in the nociceptive synapse. a, N-to-L EPSPs before and after T-cell LFS (top). No depression was observed in control experiments in which the LFS was omitted (middle; black traces represent pretest EPSPs, and the gray traces represent the posttest EPSP recordings). Each EPSP was elicited by a single N-cell action potential (bottom). b, Significant depression was observed at the 0, 30, 60, and 120 min after LFS. *p < 0.01, one-way ANOVA with Newman–Keuls post hoc analyses. Each time point in the control and LFS groups represents a separate group of synapses that were tested. c, Pretest recordings of the nociceptive synapse was followed by 15 min of LFS, 2-AG, or capsaicin treatment. Posttest recordings of the N-to-L pathway occurred immediately after LFS or treatment had ended. d, Bar graph representing nociceptive depression observed immediately after the 15 min LFS, 2-AG, or capsaicin treatment. Significant depression was observed in each treatment group compared to control data. *p < 0.001, one-way ANOVA with Newman–Keuls post hoc. Data presented as normalized mean ± SEM.