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. 2013 Mar 6;33(10):4349–4358. doi: 10.1523/JNEUROSCI.3922-12.2013

Figure 3.

Figure 3.

Protein and RNA synthesis is necessary for eCB–LTD in the nociceptive synapse (60 min posttest). a, Left, Traces showing the decrease in EPSP amplitude after T-cell LFS in vehicle (0.01% DMSO in saline), whereas no depression was observed when emetine was applied during LFS. Right, Bar graph comparing the effects on EPSP amplitude in the control, LFS, LFS + emetine, and emetine-treated groups. Depression normally observed after LFS treatment was blocked when emetine was applied (15 min) during LFS. Emetine alone had no effect. b, Left, Traces showing a decrease in EPSP amplitude after LFS but no depression when LFS was delivered in the presence of the bath-applied transcription inhibitors actinomycin D (actino) or DRB. Right, Bar graph comparing the effects on EPSP amplitude after no treatment, LFS alone, LFS + actinomycin D, actinomycin D alone, LFS + DRB, and DRB alone. Both actinomycin D and DRB blocked LFS-induced depression. No effect on EPSP amplitude was observed when actinomycin D or DRB was applied without LFS. *p < 0.01, one-way ANOVA with Newman–Keuls post hoc. Data presented as normalized mean ± SEM.

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