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. Author manuscript; available in PMC: 2014 Mar 15.
Published in final edited form as: J Immunol. 2013 Feb 8;190(6):2501–2509. doi: 10.4049/jimmunol.1201660

Figure 3. The lack of correlation between Kb-SIINFEKL surface expression and protein half-life is not limited to chimeric nucleoprotein constructs.

Figure 3

(A) The NP moiety of AU-NP-S-FLAG and AU-R-NP-S-FLAG was replaced with the amino-terminal portion of influenza acid polymerase (PA) to generate AU-PA-S-FLAG or AU-R-PA-S-FLAG. (B) BMA3 cells were infected with Listeria expressing AU-PA-S-FLAG or AU-R-PA-S-FLAG in the presence or absence of 5 µM expoxomicin. At 3 h post-infection, cells were lysed and immunoblotted for FLAG and β-actin. FLAG staining was normalized to β-actin for three independent experiments. Data are shown as mean ± SEM, *,p<0.05. (C) BMA3 cells, (D) BMMΦ, and (E) BMDC were infected with Listeria expressing AU-PA-S-FLAG or AU-R-PA-S-FLAG and surface Kb-SIINFEKL levels were measured at 20 min intervals from 80 to 300 min post-infection. Graphs are representative of 3 independent experiments.