Figure 5. Co-infection with Listeria and rVV does not alter K^b-SIINFEKL presentation kinetics from either vector.

(A) Surface K^b-SIINFEKL levels were determined for BMA3 cells infected with rVV expressing NP-S-eGFP alone or BMA3 cells co-infected with Listeria not expressing the SIINFEKL peptide. (B) Surface K^b-SIINFEKL levels were determined for BMA3 cells infected with Listeria expressing AU-NP-S-FLAG alone or BMA3 cells co-infected with rVV not expressing SIINFEKL peptide. In both infections, Listeria expressed the red fluorescent mCherry protein and rVV expressed either eGFP (A) or Venus (B) to limit K^b-SIINFEKL analysis to BMA3 cells infected with both vectors. Each graph is representative of 3 independent experiments.