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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2012 Mar 15;32(5):1124–1131. doi: 10.1161/ATVBAHA.111.239558

Figure 4. Aorta protein expression and insulin signaling.

Figure 4

A, Representative Western blots (top left) based on lysate of aorta. Graphs show average protein expression calculated from densitometry of Western blots based on tissue from 6 or 8 animals in each group, except for ET-1, where quantitation was based on 5 animals in each group. B, Insulin (50 mU/g) or insulin diluent was injected into the vena cava during pentobarbital anesthesia. After 5 minutes, the aorta was isolated and flash frozen. Representative Western blots (top) based on lysate of aorta. Average expression (bottom) calculated from densitometry of Western blots based on tissue from 3 animals in each group (total of 6 control (c) and 6 haploinsufficient (Δ) mice, Insr flox/ flox Apoe−/− and Insr flox/Δ Apoe−/−, respectively).