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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Proteome Res. 2008 Feb 27;7(4):1490–1499. doi: 10.1021/pr700804c

Figure 5.

Figure 5

Development of custom tumor antigen high-density programmable protein microarrays for the detection of autoantibodies in patient sera. A) Over 1,700 candidate tumor antigens were expressed and captured in a microarray format, and protein expression was detected using anti-GST antibody. B. The arrays were probed with sera from a healthy individual, and patients with melanoma, breast and ovarian cancer. All arrays were incubated with 1:300 diluted serum and developed using a HRP conjugated anti-human IgG and TSA. (i) Shows EBNA response from healthy serum (red circle, CV=40% across 4 slides) (ii) Response to ML-IAP from melanoma serum (CV not tested) (iii) Response to p53 from breast cancer sera (CV=11% across 4 slides) (iv) Response to p53 from ovarian cancer sera (CV=49% across 2 slides).