Figure 2.

Western blotting analysis of shellfish extracts with anti-OABP2.1 serum. (A–E) Extracts were prepared from the nontoxic scallop Mizuhopecten yessoensis (A, B), the toxic scallop M. yessoensis (C, D), the toxic Mytilus galloprovincialis (E, F) and toxic ascidian Halocynthia roretzi (G). The pellet fractions were A, C and E. The supernatant fractions were B, D and F. The whole homogenate was used for H. roretzi. Lanes 1–6 correspond to recombinant OABP2.1, gills, gonad, adductor muscle, digestive gland and mantle, respectively. (H, I) Binding of [24-³H]OA to the pellet fraction (H) and supernatant fraction (I) of nontoxic M. yessoensis (grey bars). Protein concentration of each fraction was represented with red lines. Lane description was the same as described for A–G.