Cytotoxic effects of clerosterol in A2058 human melanoma cells and HaCaT human keratinocyte. Cell viability (a) was assessed using the MTT assay after treatment of A2058 cells with the indicated concentrations of clerosterol (20, 60, 100, 150, 200 µM) for 24 h and (b) after treatment with 150 µM clerosterol for the indicated times. * Significantly different from control cells (p < 0.05). (c) Cell viability was assessed using the MTT assay after treatment of HaCaT and A2058 cells with clerosterol and 7β-hydroxycholesterol at 150 µM for 24 h. *, ** Significantly different from control cells in HaCaT human keratinocyte and A2058 human melanoma cells (p < 0.05).