Figure 1.

Comparison of Hcrt-positive neurons and spontaneous homecage activity between AT mice and their WT littermates. Representative images of immunofluorescent staining of the lateral hypothalamus taken from WT mice (a) and AT mice (b) at 3 months of age are shown. Scale bar, 100 μm in b applied to a. Shown is a comparison of active time (c, recording hour 1,*p = 0.048, and recording hour 8, p = 0.015), traveling distance (d, recording hour 1, *p = 0.025, and recording hour 8, p = 0.014), rearing activity (e), and traveling speed (f) in AT and WT littermates in a 24 h record using the SmartCage system. Gray background in figures c–f indicates dark phase during a 12:12 light:dark cycle (lights on at 7:00 A.M.; lights off at 7:00 P.M.). Data shown are mean ± SEM for each time point (1 h bin). Subjects were 5-month-old male mice (n = 8/genotype).