Table 1.
Gene targets and controls used to assess macrophage phenotype by RT-PCR.
| Gene Symbol | Gene Name, Entrez Gene ID | Higher in Mo/M1/M2 |
|---|---|---|
| CD11b | Itgam, Integrin, alpha M, 3684 | M2 |
| CD11c | Itgax, Integrin, alpha X, 3687 | M1 |
| CD36 | Leukocyte differentiation antigen, 948 | M2 |
| CD68 | CD68 molecule, 968 | M1 & M2 |
| CD163 | CD163 molecule, 9332 | M2 |
| CD206 | Mannose receptor C type 1, MRC1, 4360 | M2 |
| CCL19 | Chemokine ligand 19, MIP-3b, 6363 | M1 |
| CCL20 | Chemokine ligand 20, MIP-3a, 20297 | M1 |
| CCR2 | Chemokine receptor 2, CD192, MCP-1R, 729230 | Mo |
| CCR7 | Chemokine receptor 7, CD197, 1236 | M1 |
| CXCL1 | Chemokine (C-X-C motif) ligand 1, NAP-3, 2919 | M1 |
| CXCL3 | Chemokine (C-X-C motif) ligand 3, MIP-2b, 2921 | M1 |
| CX3CR1 | Chemokine (C-X3-C motif) receptor 1, 1524 | Mo |
| IL-1b | Interleukin 1, beta; IL1F2, 3553 | M1 |
| IL-6 | Interleukin 6 (interferon, beta 2), 3569 | M1 |
| IL-7R | Interleukin 7 receptor, CD127, IL7RA, 3575 | M1 |
| IL-8 | Interleukin 8, CXCL8, NAP1, 3576 | M1 |
| IL-12 | Interleukin 12A, p35, 3592 | M1 |
| IL-23 | Interleukin 23, alpha, 51561 | M1 |
| Arg 1 | Arginase, liver, 383 | M2 |
| BCL2A1 | BCL2-related protein A1, 597 | M1 |
| COX2 | Prostaglandin-endoperoxide synthase 2, PTGS2, 5743 | M1 |
| BIRC3 | Baculoviral IAP repeat-containing 3, 330 | M1 |
| CSF-1 | Colony stimulating factor 1 (MF), M-CSF, 1435 | M2 |
| CSF-2 | Colony stimulating factor 2 (granulocyte-macrophage), GM-CSF,1437 | M1 |
| EDN1 | Endothelin 1,1906 | M1 |
| F4/80 | Zinc finger protein 808, EMR1, 2015 | M1 & M2 |
| FGL2 | Fibrinogen-like 2, fibroleukin,10875 | M2 |
| FN1 | Fibronectin 1,2335 | M2 |
| HS3ST1 | Heparan sulfate (glucosamine) 3-O-sulfotransferase 1, 9957 | M1 |
| HSD11B1 | Hydroxysteroid (11-beta) dehydrogenase 1, 3290 | M2 |
| INDO | Indoleamine 2,3-dioxygenase 1, IDO1, 3620 | M1 |
| iNOS | Nitric oxide synthase 2, inducible; NOS2A, 4843 | M1 |
| LIPA | Lipase A, lysosomal acid, cholesterol esterase, 3988 | M2 |
| MAF | v-Maf musculoaponeurotic fibrosarcoma oncogene homolog, 4094 | M2 |
| MMP14 | Matrix metallopeptidase 14, 4323 | M2 |
| MS4A4A | Membrane-spanning 4-domains, subfamily A-member 4, 51338 | M2 |
| MSR1 | Macrophage scavenger receptor 1, CD204, 4481 | M2 |
| OASL | 2′-5′-oligoadenylate synthetase-like, 8638 | M1 |
| OPN | Osteopontin; SPP1, Secreted phosphoprotein 1, 6696 | M1 |
| PTX3 | Pentraxin 3, long; TNFAIP5, 5806 | M1 |
| TNF-a | Tumor necrosis factor (superfamily member 2), 7124 | M1 |
| VEGF-a | Vascular endothelial growth factor A, 7422 | M2 |
| GAPDH | Gyceraldehyde-3-phosphate dehydrogenase, 2597 | N/A |
| WWP1 | Ubiquitin, protein ligase 1, 11059 | N/A |
| HGDC | Human Genomic DNA Contamination | N/A |
| RTC | Reverse Transcription Control | N/A |
| PPC | Positive PCR Control | N/A |