Fig. 5.

Mutational analysis of candidate Tcf sites in the blimp1 cis-regulatory module. The table shows spatial reporter expression of blimp1 BAC-GFP, construct 27, and construct 27 with both Tcf sites mutated. (A, B) Diagrams depicting embryos at early mesenchyme blastula stage, and displaying the wild-type blimp1 pattern of veg₂ expression at this stage in blue, ectopic expression in the ectoderm in red. (C–F) Representative fluorescence images of transgenic embryos: (C) correct GFP reporter expression driven by construct 27; (D–F) examples of gross ectopic reporter expression driven by construct 27 in which either one or both Tcf sites had been mutated (D, Tcf site 1 mutated; E, Tcf site 2 mutated; F, both mutated).