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. Author manuscript; available in PMC: 2014 Jun 10.
Published in final edited form as: Cancer Lett. 2013 Jan 29;333(2):239–243. doi: 10.1016/j.canlet.2013.01.041

Figure 1.

Figure 1

Ablation of eIF4E expression induces apoptosis in the presence of serum in MDA-MB-231 cells. MDA-MB-231 cells were transfected at 50% confluence with negative control siRNA or siRNA for eIF4E. 24 hr later, the cells were put in fresh media with either 0% or 10% serum. After another 24, the percentage of non-viable cells was determined as described in Materials and Methods. Also at determined at this time was the level of cleaved PARP, eIF4E, and actin by Western blot analysis. The cell viability data is representative of data obtained from at least four independent experiments. The immunoblots are representative of experiments repeated at least two times.