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. Author manuscript; available in PMC: 2014 Jun 10.
Published in final edited form as: Cancer Lett. 2013 Jan 29;333(2):239–243. doi: 10.1016/j.canlet.2013.01.041

Figure 2.

Figure 2

Suppression of S6 kinase reverses the apoptotic effect of suppressed eIF4E expression. (A) MDA-MB-231 cells were transfected at 50% confluence with negative control siRNA or siRNA for eIF4E as indicated. 24 hr later, the cells were put in fresh media containing either 10% or 0% serum and the indicated concentration of rapamycin or DMSO vehicle. After another 24 hr, the level of cleaved PARP, eIF4E, P-S6K, and S6K by Western blot analysis. (B) MDA-MB-231 cells were transfected at with negative control siRNA, siRNA for eIF4E, or S6 kinase as indicated. 24 hr later, the cells were put in fresh media with 10% serum. After another 24 hr, the level of cleaved PARP, eIF4E, S6K, and actin by Western blot analysis. The data are representative of experiments repeated at least two times.