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. Author manuscript; available in PMC: 2014 Jun 10.
Published in final edited form as: Cancer Lett. 2013 Jan 29;333(2):239–243. doi: 10.1016/j.canlet.2013.01.041

Figure 4.

Figure 4

Suppression of S6 kinase induces G1 arrest in cells with ablated eIF4E. (A) MDA-MB-231 cells were transfected at 50% confluence with negative control siRNA or siRNA for eIF4E. 24 hr later, the cells were put in fresh media with 10% serum and the indicated concentrations of rapamycin. (B) MDA-MB-231 cells were transfected at 50% confluence with negative control siRNA, siRNA for eIF4E, or S6 kinase. 24 hr later, the cells were put in fresh media with 10% serum. The cells in both (A) and (B) were then subjected to flow cytometric analysis 24 hr later as described in Materials and Methods. The DNA content per cell shown graphically above is from a representative experiment that was repeated three times.