Figure 2.
SDF-1α and CXCR4 expression in mouse cardiomyocytes and fibroblasts. Expression of SDF-1α and CXCR4 mRNA and protein was determined in cardiac myocytes and fibroblasts isolated from adult mouse hearts. A, mRNA was detected by RT-PCR using primers described in Methods. B, Protein was detected by immunoprecipitation and immunoblotting with antibodies against SDF-1α and CXCR4 (in which lentiviral overexpression of both SDF-1α and CXCR4 in 293FT cells served as positive control).