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iNOS gene expression after rAAV-mediated gene transfer in vitro. COS-7 cells were treated with rAAV/LacZ (negative control), rAAV/iNOS, or lipofectamine-mediated pcDNA3.1/iNOS gene transfer (positive control). Four days later, proteins were isolated from cells and subjected to Western blotting with anti-murine iNOS antibody. Each experiment was repeated thrice
