Skip to main content
. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Mech Dev. 2009 Jul 10;126(10):898–912. doi: 10.1016/j.mod.2009.07.002

Figure 5.

Figure 5

Electromicrographs of transverse sections through the 10dpf larval pancreas. (A) At low power the whole transverse section of the pancreas appears triangular. Large nuclei and zymogen granules characterize acinar cells of the exocrine pancreas. Right in the middle of the pancreas the lumen of the pancreatic duct can be seen ({, scale bar = 10μms). (B) Close up of lumen region in (A). Three cells can be seen around the circumference of the lumen (scale bar = 1μm). (C) These duct localized cells have projection into the lumen. Wherever the cell membranes of these cells are in direct contact with each other, distinctive tight junctions are observed (►, scale bar = 500nm). (D) Immunoelectron microscopy using a gold conjugated secondary to detect GFP protein in a pancreas of a Tg(Tp1bglob:eGFP)um13 larva (scale bar = 500nm). The ultra-structure appears slightly different due to changes in fixation to permit immunolabelling. Gold particles appear very dark and circular (see inset enlargement) and are detected throughout the cells lining the duct. These cells are morphologically similar and in the same position as the cells previously observed by EM. This demonstrates that the previously described PNCs are located in the lining of the pancreatic duct.