Step	Notes
1	Dispensing on the PP-384-M Personal Pipettor using a custom 384 head; 30 sec per 384-well microtiter plate
2	Dispensing into assay plate with Multidrop 384
3	Dispensing into assay plate with Multidrop 384
4	Assay plates at room temperature
5	Cells prepared in media and dispensed into assay plate with Multidrop 384
6	Assay plates stored in the Cytomat; an automated incubator
7	Aspirating on the ELx405 automated washer and dispensing with Multidrop 384; 1 min per 384-well microtiter plate
8	Dispensing on the PP-384-M Personal Pipettor using a custom 384 head; 30 sec per 384-well microtiter plate
9	Assay plates stored in the Cytomat
10	Dispensing into assay plate with Multidrop 384
11	Assay plates stored in the Cytomat
12	Aspirating on the ELx405 and dispensing with Multidrop 384; 1 min per 384-well microtiter plate
13	For assay development and screening, nine images per well for 20 sec per well with total imaging time of 135 min per 384-well microtiter plate.
14	Analysis of EGFP signal and Draq5-stained nuclei, 10 min per 384-well microtiter plate