FIG. 3.

Endonuclease digestion qPCR. (a) Schematic diagram of the technique. (b) Graph of measured values for clinical-grade scAAV2/8-LP1-hFIXco preparation, when technique is performed under the conditions indicated (see Materials and Methods). Error bars represent standard deviation of replicates. (c) Quantitative PCR results for a similar scAAV preparation, when performed with the additional treatments indicated. “Plasmid spike” indicates addition of 6.8×10¹² GC/ml of linearized plasmid standard to vector test article before the procedures.