Figure 6. Akt Phosphorylation Increases in Airways Exposed to Cr(VI).
A) Bright field microscopy pictures taken of lung sections obtained from mice at 0–24 hrs post-intranasal exposure to 50 µL of 1.2 mg/mL basic zinc chromate suspended in saline. Lung sections were stained by immunohistochemistry with phosphospecific serine-473 AKT antibody and representative images were taken at 40×, original magnification. B) Quantification of phospho-Akt staining frequency and intensity in lung airways. Staining intensity was scored on a scale of 1–3, where 1 is a low level of staining and 3 is strong staining. Data are the mean ± SEM of 4 mice and * denotes a statistically significant difference from time 0 at p<0.01.