. 2013 Jan 31;5(2):550–567. doi: 10.3390/v5020550

Table 2.

Sequence and position of the primers used in RT-PCR.

Primers	Sense ^a	Sequence (5′→3′)	Position in genome ^b	Length	Gene
P1	+	ACAGAGACAAGTTGGCAYGA	20035-20054	1845 bp	Partial of S1
P2	−	CCWGAMACTACAAACTGYTG	21860-21879	1845 bp	Partial of S1
P3	+	AAGAGYGRTGGCTCTCGTA	21580-21598	1800 bp	Partial of S1 and S2
P4	−	GTAACTAYATCTCCTGCAGT	23360-23379	1800 bp	Partial of S1 and S2
P5	+	TGCACCTAATGGYATAGTGT	23158-21579	1607 bp	Partial of S2, 3a, 3b, and 3c(E)
P6	+	ACAGAGACAAGTTGGCAYGA	24745-24764	1607 bp	Partial of S2, 3a, 3b, and 3c(E)

^a Negative-sense (−) or positive-sense (+) primer; ^b The nucleotide positions correspond to those in the sequence of the IBV M41 genome (GenBank accession number DQ834384).