Table 7.
Influence of O,P and OOP-RNA expression on cell killing and plasmid loss. a
| Plasmids in 594 host cells | Cell viability and (plasmid retention/cfu; %)at growth temperature (˚C) | ||||
|---|---|---|---|---|---|
| 30 | 35 | 37 | 39 | 42 | |
| pcIpR- P-timm | 1.0 | 0.32 | 0.01 | 0.008 | 0.07 |
| (35/35; 100) | (33/33; 100) | (0/35; 0) | (0/35; 0) | (0/35; 0) | |
| pcIpR- O-P-timm | 1.0 | 0.345 | 0.12 | 0.12 | Nd |
| (62/70; 89) | (28/70; 40) | (0/70; 0) | (0/70; 0) | ||
| pcIpR- O-36P-timm | 1.0 | 0.825 | 0.793 | 0.012 | Nd |
| (30/30; 100) | (30/30; 100) | (30/30; 100) | (1/30; 3) | ||
| pcIpR- O-63P-timm | 1.0 | 0.895 | 0.895 | 0.055 | Nd |
| 30/30; 100) | (30/30; 100) | (30/30; 100) | (14/40; 35) | ||
| pcIpR- O-timm | 1.0 | 1.0 | 1.0 | 1.0 | 0.608 |
| (30/30; 100) | (30/30; 100) | (30/30;100) | (30/30; 100) | (29/30; 97) | |
| p434’pR- O-timm b | 1.0 | 1.0 | 1.0 | 1.0 | 0.615 |
| (26/30; 87) | (29/30; 97) | (28/30; 93) | (26/30; 87) | (26/30; 87) | |
| pcIpR- oop#1-O-P-timm c | 1.0 | 0.938 | 0.20 | 0.005 | Nd |
| (120/120; 100) | (120/120; 100) | (98/101; 97) | (115/120; 96) | ||
| pcIpR- oop#2 O-P-timm c | 1.0 | 0.988 | 0.055 | 0.048 | Nd |
| (117/120; 98) | (60/60; 100) | (76/154; 49) | (62/120; 52) | ||
a: As described for Table 6, except stationary phase cultures were grown up at 30 and not 25 ˚C.
b: Plasmid has constitutive expression of O.
c: The viability results represent the average for four independent plasmid isolates, each single experiments. The results in parentheses, for plasmid retention by survivor cfu, sums results for all the cfu’s assayed from the four isolates. The cell viabilities at 30 and 35 ˚C were at or very near unity. pcIpR-oop#1-O-P-timm: 37˚C (0.34, 0.49, 0.006, 0.008), 39˚C (0.01, 0.006, 0.0006, 0.0015); pcIpR-oop#2-O-P-timm: 37˚C (0.01, 0.01, 0.12, 0.08), and 39˚C (0.01, 0.01, 0.09, 0.08).