Table 8.

Contrasting trans P-lethality / inhibition and cis Replicative Killing

A. Cells tolerate short-term exposure to P (short-term interference, not lethality) a
Strain with plasmid	Incubation at 37 ˚C	Cell viability (AmpR cfu/ total cfu)	Outcome of P expression from plasmid
594[pcIpR-P-timm]	1 hr	0.87 (232/242)	most cells recover
	2 hr	0.49 (258/281)	many cells recover
	6 hr	0.13 (2/173)	high plasmid loss
B. Inducing replication from a trapped cryptic prophage causes Replicative Killing a
Strains with cryptic prophages	Prophage Induction time	Cell viability	Outcome of prophage Induction
Y836[~cIII-cI857-O-P-ren]	5 min	0.33	rapid cell killing
	20 min	0.13	rapid cell killing
	3 hr	0.00018	extensive cell killing
	5 hr	0.00008	extensive cell killing
594[~cIII-cI857-O-P-ren]	5 min	0.55	rapid cell killing
	20 min	0.25	rapid cell killing
	3 hr	0.0022	extensive cell killing
	5 hr	0.0022	extensive cell killing
Y836[~cIII-cI857-O-P::kan-ren]	3 hr	5.1 b	cell growth
	5 hr	6.1 b	cell growth

^a: Refer to Experimental Section 3.6.

^b: The increase in viability by for example 5.1 indicates somewhat more than two and less than three cell doublings.