Table 2.
Inhibition of the bacterial growth (incubation time 6 h) in LB medium at 30 °C supplemented by svPLA2s (500 μg/mL, i.e., 36.2 μM for VBBPLA2; 37.8 μM for NNOPLA2; 36.5 μM for VLPLA2) from three different snakes.
| Inhibition of the bacterial growth, % (t = 6 h) | ||||||
|---|---|---|---|---|---|---|
| Bacteria(Gram staining) | svPLA2 | |||||
| V. b. berus | V. b. berus * | V. lebetina | V. lebetina * | N. n. oxiana | ||
| Escherichia coli | Gram (−) | No effect | not tested | No effect | No effect | No effect |
| Bacillus subtilis | Gram (+) | 100% ** | 99% ** | 13% | No effect | Slight effect (6.5%) |
| Staphylococcus aureus | Gram (+) | No effect | 29% | No effect | No effect | 42% |
* histidine in PLA2 was modified by p-bromophenacylbromide, to inactivate its catalytic activity; ** growth was inhibited by 100% but the viability of bacteria remained unchanged (i.e., after the 6 h exposure to enzyme preparation, bacteria were able to grow on agarized LB-medium; data not shown).