Table 2.

ω-ConotoxinCa_v2.2 blockers: Sequence, indicating conserved cysteine residues in bold face type and potency at ¹²⁵I-GVIA or MVIIA binding assays.

ω-conotoxin name	ω-conotoxin Sequence	125I-Ctx binding assays to rat brain IC50/Kd (nM)	Reference
CnVIIA	CKGKGAOCTRLMYDCCHGSCSSSKGRC*	0.4 (2.2 > 2.1)	[82]
CVIA	CKSTGASCRRTSYDCCTGSCRSGRC	0.6 (2.2 > 1.2)	[8]
CVIB	CKGKGASCRKTMYDCCRGSCRSGRC	7.7 (2.2~2.1 > 2.3)	[8]
CVIC	CKGKGQSCSKLMYDCCTGSC-SRRGKC	7.6 (2.1~2.2)	[8]
CVID	CKSKGAKCSKLMYDCCSGSCSGTVGRC	0.07 (2.2 > 2.1)	[8]
CVIE	CKGKGASCRRTSYDCCTGSCRSGRC	0.025 (2.2 > 2.1 > 1.2~1.3~2.3	[16]
CVIF	CKGKGASCRRTSYDCCTGSCRLGRC	0.098 (2.2 > 2.1 > 1.2~1.3~2.3)	[16]
FVIA	CKGTGKSCSRIAYNCCTGSCRSGKC	ND (2.2 > 2.1 > 3.2)	[83]
GVIA	CKSOGSSCSOTSYNCCRSCNOYTKRCY*	0.04 (2.2 > 2.1)	[84,85,86,87,88]
GVIB	CKSOGSSCSOTSYNCCR-SCNOYTKRCYG*	ND	[88,89]
GVIIA	CKSOGTOCSRGMRDCCTSCLLYSNKCRRY*	3.7 (ND)	[88,89,90]
GVIIB	CKSOGTOCSRGMRDCCTSCLSYSNKCRRY*	ND	[88]
MVIIA	CKGKGAKCSRLMYDCCTGSCRSGKC	0.055 (2.2 > 2.1)	[13,81,87]
RVIA	CKPPGSPCRVSSYNCCSSCKSYNKKCG	0.25 (2.2)	[10]
TVIA	CLSXGSSCSXTSYNCCRSCNXYSRKCR	ND (2.2 > 2.1)	[91,92]

Source: Conoserver database: www.conoserver.org. ¹²⁵I-Ctx = ¹²⁵I-GVIA or ¹²⁵I-MVIIA displacement assays to define ω- conotoxins binding to Ca_v2.2 expressed in different brain preparations including rat, chicken and mouse brain. ND= Not determined; in brackets the order of Ca_v type selectivity for each ω-conotoxin is described.* O=hydoxyproline (PTM: post-translational modification).