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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Orthop Res. 2012 Jun 13;30(12):1979–1984. doi: 10.1002/jor.22172

Figure 2. PTH-induced GJIC is age-related and independent of de novo Gja1 or Cx43 expression.

Figure 2

Figure 2

Figure 2

Osteoblastic cells were isolated and GJIC was evaluated as described in Materials and Methods. (A) Recombinant PTH(1-34) dose-dependently increased the degree of GJIC in osteoblastic cells isolated from young, but not mature or old, rats. (B) Recombinant PTH(1-34) (10−11–10−7 M) had no effect on Gja1 mRNA expression in osteoblastic cells from young, mature, or old rats. (C) Recombinant PTH(1-34) (10−11–10−7 M) had no effect on Cx43 protein expression in osteoblastic cells from young, mature, or old rats.. a indicates p < 0.05 compared to osteoblastic cells from mature rats; b indicates p < 0.05 versus osteoblastic cells from old rats; c indicates p < 0.05 young treated versus control. n=2-4 animals per age group per treatment.