Fig. 5.
Study the damaged DNA. (A) SDS-PAGE analysis of the methanol-insoluble cell extract and medium fractions of RKO/TREX1 cells, followed by auto-radiogram. λ/HindIII markers stained by ethidium bromide and γ-[32P]ATP labeled 21mer oligonucleotide used in the exonuclease activity assay were used as the size markers. (B) HPLC analysis of the methanol-soluble fraction of medium. (Thy, thymine; Thd, thymidine; TMP, thymidine monophosphate; TDP, thymidine diphosphate; TTP, thymidine triphosphate). (C) Immunoblot analysis.