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. Author manuscript; available in PMC: 2014 Apr 25.
Published in final edited form as: Cell Rep. 2013 Apr 4;3(4):1028–1035. doi: 10.1016/j.celrep.2013.03.011

Figure 3.

Figure 3

DR8 directed oligonucleotides alter the splicing of endogenous telomerase.

(A) The direct repeats DR6 and DR8 are 85% identical and are members of the J1 family of Alu repeats. The sequence of the 20bp 2-O-methyl oligonucleotide containing 50% thiophosphate linkages complementary to the region starting at nt19 is boxed. (B) Introduction of the oligonucleotide DR8+19 dramatically shifts splicing of the endogenous telomerase mRNA towards the production of the non-functional minus beta isoform. Cells were treated with 100nM DR8+19 oligonucleotide or vehicle control. Right side shows the quantification.