Figure 3. Uba6^NKOmice display social interaction deficits.

(A) Uba6^NKO mice (female) exhibit impaired nest-building behavior. Mice were habituated in an empty cage for 15 min, and a 5 × 5 cm square of pressed cotton was placed in a random cage corner. The width and height of the nest built by the mouse, with subtraction of the original width and height of the cotton pad, was measured at 30, 60, 90 min and 48 h. All values are mean ± SEM. **P < 0.01, ***P < 0.001. Control (n=8) and Uba6^NKO (n=8).

(B-D) Uba6^NKO mice (female) exhibit social interaction defects, as determined by percentage of total interaction time with stranger in three-chamber social interaction test (B) and the time with the stranger enclosure (C-D). Dashed line in (B) represents chance-level performance (i.e. 50%) when mice equally explore the chamber containing a stranger mouse and the opposite chamber. Error bars are mean ± SEM and the statistical significance between control (n=8) and Uba6^NKO (n=6) mice is **P < 0.01.

(E) Forced swim test. Uba6^NKO (n=6) mice displayed significantly less immobility time than Control (n=8) mice (all female). All values are mean ± SEM. **P < 0.01. Also see Figure S2 and S3.