Figure 6. Alisertib treatment inhibits AURKA activation in tumors.
Tumor samples from control and drug-treated mice were subjected to immunohistochemical or electrochemiluminescent ELISA detection of pAURKAT288. A) Immunohistochemical detection of pAURKAT288 in tumor sections. The same exposure time was used to capture all images and the scale bar = 50 µm. B and C) ELISA detection of pAURKAT288 in total tumor protein lysates showed significantly lower levels of AURKA activation in mice treated with alisertib alone or in combination with paclitaxel compared to vehicle-treated controls (**P < 0.01).