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Effects of hfq mutations on sRNA levels under specific growth conditions. Extracts were prepared from wild-type and mutant derivatives of SG30200 (PBAD-rpoS-lacZ) grown in LB medium at 37 °C to early stationary phase (OD600 ~ 1.0). ArcZ levels were analyzed by Northern blots while the levels of all other sRNAs were analyzed by primer extension. In both cases, 5 µg of total RNA and primers specific to the indicated RNAs were used.
