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. Author manuscript; available in PMC: 2014 Oct 9.
Published in final edited form as: J Mol Biol. 2013 Jan 11;425(19):3678–3697. doi: 10.1016/j.jmb.2013.01.006

Fig. 5.

Fig. 5

Effects of hfq mutations on Hfq association with select (a) sRNAs and (b) mRNAs. Extracts were prepared from wild-type and hfq mutant derivatives of SG30200 (PBAD-rpoS-lacZ) cells grown in LB medium at 37 °C to early stationary phase (OD600 ~ 1.0). co-IP was carried out with anti-Hfq antiserum. ArcZ levels were analyzed by Northern blots while the levels of all other RNAs were analyzed by primer extension. In both cases, 5 µg of total RNA or 0.5 µg of co-IP RNA and primers specific to the indicated RNAs were used.