Table 1. Summary of in vivo assays.

The plate and growth assays shown in Fig. 2a, 2c, 2e, 3a, 3c, 3e and 3g are summarized here. Hfq alleles are given on the far left of the table. The residues investigated in the study from the three faces of Hfq are grouped in the table as proximal (red), rim (purple), and distal (blue). The top line of the table describes the assay in which the given Hfq allele was examined. Results for each Hfq allele for a given assay are reported as follows: green = an allele was functional for a given assay, yellow = an allele showed partial function, or orange = an allele was defective.

Hfq allele	DsrA/rpoS	ArcZ/rpoS	McaS/flhD	ArcZ/flhD	ChiX/chiP	RyhB/sodB	RyhB/sdh
	(activity on lac plates)	(activity on lac plates)	(activity on lac plates)	(activity on lac plates)	(activity on lac plates)	(Northern)	(growth on succinate)
wt	Functional	Functional	Functional	Functional	Functional	Functional	Functional
hfq-	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional
Q8A	Functional	Nonfunctional	Functional	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional
D9A	Functional	Functional	Functional	Functional	Nonfunctional	Partial	Nonfunctional
F39A	Functional	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional	Partial
F42A	Functional	Partial	Functional	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional
K56A	Partial	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional	Nonfunctional
H57A	Functional	Functional	Functional	Functional	Functional	Functional	Partial
R16A	Functional	Partial	Nonfunctional	Nonfunctional	Nonfunctional	Functional	Functional
R16C	Functional	Partial	Nonfunctional	Nonfunctional	Nonfunctional	Functional	Functional
R17A	Functional	Functional	Nonfunctional	Functional	Nonfunctional	Functional	Functional
R19D	Functional	Functional	Nonfunctional	Functional	Nonfunctional	Functional	Functional
Y25D	Nonfunctional	Nonfunctional	Nonfunctional	Functional	Partial	Functional	Functional
G29A	Functional	Functional	Functional	Functional	Functional	Functional	Functional
I30D	Functional	Nonfunctional	Nonfunctional	Functional	Partial	Partial	Partial
K31A	Functional	Functional	Functional	Functional	Functional	Functional	Functional