Table 2. Summary of β-galactosidase assays for cell growth in low and high arabinose.

Representative alleles from each face of Hfq were examined by β-galactosidase assays (Fig. 2b, 2d, 2f, 3b, 3d, 3f, and 3h). Hfq alleles and color-coding are as for Table 1. The levels of L-arabinose used in each assay are depicted as percentages. The percentage activity for each allele was calculated as follows: positively-regulated targets: (Miller units of hfq allele − Miller units of Δhfq)/(Miller units of wild-type hfq − Miller units of Δhfq) × 100% = percent function for Hfq allele; negatively-regulated targets: (Miller units of Δhfq − Miller units of hfq allele)/(Miller units of Δhfq − Miller units of wild-type hfq) × 100% = percent function for Hfq allele. Activities of 75% or more are colored green, activities between 50% and 74% are colored yellow, and activities <50% are colored orange. β-galactosidase values for each assay are found in Supplemental Table S1.

Hfq allele	ArcZ/rpoS	ArcZ/rpoS	McaS/flhD	McaS/flhD	ArcZ/flhD	ArcZ/flhD	ChiX/chiP	ChiX/chiP	RyhB/sodB	RyhB/sodB	RyhB/sdhC	RyhB/sdhC
% ara	0.0002%	0.02%	0.0002%	0.02%	0.0002%	0.02%	0.0002%	0.02%	0.0002%	0.02%	0.0002%	0.02%
wt	100%	100%	100%	100%	100%	100%	100%	100%	100%	100%	100%	100%
hfq-	0%	0%	0%	0%	0%	0%	0%	0%	0%	0%	0%	0%
Q8A	<0%	<0%	45%	62%	45%	61%	59%	65%	13%	<0%	11%	51%
D9A	176%	85%	68%	87%	64%	94%	75%	80%	81%	76%	81%	70%
R16A	<0%	<0%	<0%	<0%	71%	57%	64%	47%	97%	95%	97%	96%
Y25D	34%	95%	<0%	47%	54%	77%	83%	81%	77%	55%	23%	21%
K31A	34%	66%	2%	76%	96%	89%	100%	94%	96%	83%	92%	72%