Fig. 3.
ACTH promotes cell proliferation of rat BMSC enriched for mesenchymal progenitors in a dose-dependent manner (a). γ2–MSH (b) and α-MSH (c) do not exhibit this effect. Proliferation in response to melanocortin peptides was assessed using MTT assay. The fold change in OD of formazan product from 24 h post-plating (day 0) is shown. Data are presented as means ± SD, n = 8. Data are representative of 3 separate experiments with similar results. Individual comparisons were made after a significant two-way ANOVA (day×ACTH dose) using the Bonferroni correction. a-significantly different from 0 nM, b-significantly different from 0 and 10−8 M, c-significantly different from 0, 10−8 and 10−7 nM
