Fig. 5.
ACTH enhances early gene markers of chondrogenic differentiation in rat BMSC enriched for mesenchymal progenitors. Real-time reverse transcription PCR was carried out on cultures after 4 or 9 days of culture in the presence of 10−7 M ACTH. The graph shows expression of Sox9 and Col2a1 transcripts in ACTH cultures relative to control. Expression in both groups was first normalized to a GAPDH internal control. Data are presented as mean ± SD, n = 3.
