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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Cell Microbiol. 2013 Jan 20;15(7):1127–1142. doi: 10.1111/cmi.12103

Figure 2. Endogenous gal3 plays a role in neutrophil phagocytosis of C. parapsilosis yeast and C. albicans hyphae.

Figure 2

Results are mean ± SEM of at least three separate neutrophil donors. *p ≤ 0.05 compared to untreated controls (CT) unless otherwise indicated. (A) Phagocytosis rates of C. parapsilosis after neutrophil treatment with 25 μg blocking antibodies at an E:T ratio of 1:10. Blocking antibodies were specific for gal3, toll-like receptor 2 (TLR2), TLR4, and TLR6. Three antibodies were used to block complement receptor 3 (CR3) including antibodies against CD11b domains and CD18. IgG1 and IgG2 were isotype controls. (B) Phagocytosis rates of C. albicans yeast or hyphal forms after neutrophil treatment with a blocking antibody against gal3 at indicated doses at an E:T ratio of 1:40 or 1:5, respectively. (C) Phagocytosis rates of C. albicans yeast or hyphae at various E:T ratios. * p ≤ 0.05 comparing yeast and hyphae at that E:T ratio. (D) Phagocytosis rates of C. albicans hyphae after neutrophil treatment with 25 μg blocking antibodies at an E:T ratio of 1:5. Blocking antibodies were specific for TLR2, TLR4, TLR6 and dectin-1 (Dect-1). Three antibodies were used to block complement receptor 3 (CR3) including antibodies against CD11b domains and CD18. ns=not significant. n=9 independent experiments for CT, TLR2, CR3 and Dect-1 and n=4 for TLR2.