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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Am J Transplant. 2013 Feb 22;13(5):1159–1167. doi: 10.1111/ajt.12180

Fig. 8. QMAD does not increase TGFβ production by naïve CD4 cells but decreases T cell AKT phosphorylation.

Fig. 8

(A) TGFβ measured by ELISA in Treg induction culture supernatants (with IL-2 ± QMAD) at 24 hours and 48 hours, compared to 5 day culture supernatants with TGFβ, performed in duplicate or triplicate. *p<0.05 IL-2 + TGFβ compared to all groups, n=4. (B) Representative histograms of CFSE dilution and intracellular p-AKT (assessed using monoclonal antibody) of enriched CD3+ T cells stimulated for 3 days with anti-CD3 or anti-CD3/CD28 ± QMAD. (C) Quantified results of multiple experiments performed in duplicate or triplicate (expressed as means of % of untreated control, normalized to 100%, ± SEM). *p<0.05.