Table 2.
Bait (GFP-tagged) |
Description and localization |
Species and sample type |
Optimized lysis buffer | References |
---|---|---|---|---|
Apl1 | beta-adaptin of AP-2 complex; cellular membrane | S. cerevisiae cells | 20 mM K-HEPES, pH 7.4, 110 mM KOAc, 2 mM MgCl2, 0.1% Tween, 0.4% Triton, 200 mM NaCl, 1/100 (v/v) protease inhibitor mixture (20 mg/mL PMSF + 0.4 mg/mL pepstatin A), and 1/200 (v/v) protease inhibitor cocktail | (2) |
Nup37 | Member of nuclear pore complex; subunit of Nup107–160 subcomplex | Homo sapiens cells | 20 mM K-HEPES, pH 7.4, 110 mM KOAc, 2 mM MgCl2, 0.1% TWeen, 0.5% Triton, 200 mM NaCl, 1/100 (v/v) protease inhibitor mixture (20 mg/mL PMSF + 0.4 mg/mL pepstatin A), and 1/200 (v/v) protease inhibitor cocktail | (2) |
HDAC5 | Histone deacetylase 5; nucleus and cytoplasm | Homo sapiens cells | 20 mM HEPES-KOH, pH 7.4, 0.1 M potassium acetate, 2 mM MgCl2, 0.l% Tween-20, 1 mM ZnCl2, 1 mM CaCl2, 0.5% Triton X-100, 250 mM NaCl, 4 mg/ml DNase, 1/100 (v/v) protease, and phosphatase inhibitor cocktails | (7) |
PAP I | poly (A) polymerase I; cytoplasm and inner membrane | Escherichia coli cells | 20 mM HEPES, pH 7.4, 0.11 M KOAc, 2 mM MgCl2, 0.1% Tween-20 (v/v), 1 mM ZnCl2 1 mM CaCl2, 1% Triton X-100, 0.5% Deoxycholate, 150 mM NaCl, 1/100 protease inhibitor cocktail, 1/200 phenylmethylsulphonyl fluoride | (6) |
H3 | Histone 3 isoforms; nucleus | Mus musculus ES cells | 20 mM K-HFPES, pH 7.4, 110 mM K-acetate, 0.1% Tween 20, 0.5% Triton, 300 mM NaCl, and 1/100 (v/v) protease inhibitor cocktail | (5) |
nsP3 | Sindbis nonstructural protein 3; cytoplasm | Sindbis-infected Rattus Norvegicus cells | 20 mM K-HEPES, pH 7.4, 110 mM KOAc, 2 mm MgCl2, 0.1% Tween 20, 1% Triton, 0.5% deoxycholate, 500 mM NaCl, 25 units/mL DNase, 1/100 (v/v) protease inhibitor mixture | (3) |
PSD (via VGluRd2) | postsynaptic: densities; cerebellar excitatory synapses | Mus musculus tissue | 10 mM HEPES, pH 7.4, 2 mM CaCl2, 132 mM NaCl, 3 mM KCl, 2 mM MgSO4, 1.2 mM NaH2PO4, 0.5% Triton X-100, 1/100 (v/v/) protease inhibitor cocktail | (8) |