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. Author manuscript; available in PMC: 2014 Mar 1.
Published in final edited form as: Respir Med. 2012 Nov 26;107(3):472–475. doi: 10.1016/j.rmed.2012.11.006

Figure 1.

Figure 1

SFN supplementation induces SLPI secretion in vivo and in vitro and is mediated by Nrf2. (A) Nasal lavage fluid obtained from 12 healthy volunteers on days 1–3 after daily BSH ingestion was analyzed for SLPI protein levels. (B) Densitometric analysis of SLPI expression in apical washes of NEC treated with SFN and normalized to levels in vehicle treated cells from n = 5 different subjects. Representative Western blot of SLPI levels in apical washes from NEC treated with vehicle (Veh) or SFN for 24 h (insert). (C) BEAS-2B cells were transfected with SLPI-promoter reporter plasmid, exposed to 10 µM for 5 h, and evaluated for SLPI gene transcription. SLPI-promoter reporter dependent luciferase activity was normalized to Renilla levels and expressed as relative luciferase units (RLU). In some experiments, BEAS-2B were transduced with lentiviral vectors encoding Nrf2-specific shRNA (LV-Nrf2) or scrambled vectors (LV-Scr) 48 h prior to transfection with SLPI-reporter plasmid and exposed to 10 µM for 5 h to evaluate SLPI-promoter reporter dependent luciferase. *p < 0.05, **p < 0.01, ***p < 0.001.